Viewing NGS results
To view individual annotated sequences, select an NGS analysis v2 output file and click the Sequences tab in the viewer. Upon selecting an NGS result, a loading screen with a spinner will appear and upon completion of loading the sequences will appear in batches of 1000 sequences.
The NGS results can also be sliced and diced using the Group by operation. For example, if you are interested in looking at the CDR3 region alone, you can group click Group by and depending on your data, select either Heavy CDR3, Light CDR3 or Heavy-Light CDR3 in the dropdown.
In addition to Grouping, you can also search and filter your NGS result for particular sequences within the entire NGS results or within the Group. To filter within a Group, you have to Group the sequences prior to filtering. Please refer to the following article to learn more about filtering.
These methods of Grouping and Filtering enables you to break down a large body of information into smaller groups allowing the information to be presented in a more useful manner.
Extracting annotated sequences
To extract sequences from an NGS result, select the NGS result file, click the Sequences tab in the viewer. Select the sequences that you wish to extract by selecting the checkbox and click Extract.
You can choose to either extract each individual sequences as individual sequences (outputs one document per sequence) or extract all selected sequences into a single sequence list (outputs one document for all sequences). Click Run to start the sequence extraction operation. This operation will output extracted sequences or sequence list to the same experiment folder where the NGS result file is located.
Note that sequence extraction only works with the NGS v2 pipeline output files (File type: NGS result).