To assemble a small number of Sanger sequencing reads (i.e. forward and reverse reads of the same sequence), firstly select all of the sequences you wish to assemble then click Pre-processing in the toolbar and choose Batch assemble sanger sequences.
If you have selected several groups of fragments which are to be assembled separately, you can specify a delimiter and an index at which the identifier can be found in all of the names. Sequences are grouped according to the identifier and each group is assembled separately. If a reference sequence is specified, it is used for all groups. e.g. For the names A01_M13F.ab1, A01_M13R.ab1, B02_M13F, B02_M13R.ab1, “A01” and “B02” are the identifiers, you would choose 1st for the Name part and _ (underscore) for the Name separator.
Choose the options you require and click Run to begin assembling the contig. Once complete, one or more contigs may be generated and an Assembly Report will also be generated. To view the Assembly Report, select the report and click the Assembly Report Viewer tab.
Note: The orientation of fragments will be determined automatically, and they will be reversed.